Journal: Frontiers in Cellular Neuroscience

Article Title: Neuroinflammation aggravated by traumatic brain injury at high altitude is reversed by L-serine via NFAT1-mediated microglial polarization

doi: 10.3389/fncel.2023.1152392

Figure Lengend Snippet: Effects of L-serine on inflammatory mediators, NFAT1 expression and microglial polarization after hypoxia treatment in vitro . (A,B) Levels of NO and TNF-α in LPS-treated microglia with or without different concentrations of L-serine. (C,D) Messenger RNA levels of CD16 and CD206 in cultured microglia after treatment with hypoxia in vitro with or without L-serine. (E) Protein expression levels of NFAT1 detected by Western blot in cultured microglia after treatment with hypoxia in vitro with or without L-serine. (F) Photomicrographs showing the morphology of mouse primary microglia in the three groups. n = 8–10. * p < 0.05, ** p < 0.01 versus the indicated group; # p < 0.01 versus other groups.

Article Snippet: According to a previous protocol , the sections were incubated with one of the following primary antibodies in blocking solution at 4°C overnight: rabbit antibody to myelin basic protein (MBP, 1:200, ab40390, Abcam); mouse anti-nonphosphorylated neurofilament protein (SMI-32, 1:200, NE1023, Millipore); rabbit anti-ionized anti-calcium binding adaptor molecule-1 protein (Iba-1, 1:5,000, 019–19,741, Wako); mouse anti-nuclear factor of activated T-cell 1 protein (NFAT1, 1:2,000, ab59256, Abcam); mouse anti-CD16 antibody (CD16, 1:200, BD Corporation); and mouse anti-CD206 antibody (CD 206, 1:200, R&D Corporation).

Techniques: Expressing, In Vitro, Cell Culture, Western Blot

Journal: Frontiers in Cellular Neuroscience

Article Title: Neuroinflammation aggravated by traumatic brain injury at high altitude is reversed by L-serine via NFAT1-mediated microglial polarization

doi: 10.3389/fncel.2023.1152392

Figure Lengend Snippet: Effects of L-serine on the polarization of microglia in WT mice and Nfat1 knockout mice after HA-TBI. (A) Representative images of double immunofluorescence staining of Iba-1 and CD16 in sections 0.5 mm anterior to the bregma in different groups 7 days post HA-TBI. (B) Representative images of double immunofluorescence staining of Iba-1 with CD206 at the section 0.5 mm anterior to the bregma in different groups 7 days post HA-TBI. (C) Relative cell counts of colabelled CD16 + /Iba-1 + cells in the lesion sites shown in A. (D) Relative cell counts colabelled for CD206 + /Iba-1 + in the lesion sites as shown in B. n = 6; Scale = 50 μm. ** p < 0.01 versus the indicated group; # p < 0.01 versus the HA-TBI group of WT.

Article Snippet: According to a previous protocol , the sections were incubated with one of the following primary antibodies in blocking solution at 4°C overnight: rabbit antibody to myelin basic protein (MBP, 1:200, ab40390, Abcam); mouse anti-nonphosphorylated neurofilament protein (SMI-32, 1:200, NE1023, Millipore); rabbit anti-ionized anti-calcium binding adaptor molecule-1 protein (Iba-1, 1:5,000, 019–19,741, Wako); mouse anti-nuclear factor of activated T-cell 1 protein (NFAT1, 1:2,000, ab59256, Abcam); mouse anti-CD16 antibody (CD16, 1:200, BD Corporation); and mouse anti-CD206 antibody (CD 206, 1:200, R&D Corporation).

Techniques: Knock-Out, Double Immunofluorescence Staining

Journal: Animals : an Open Access Journal from MDPI

Article Title: Pathological Study of Facial Eczema (Pithomycotoxicosis) in Sheep

doi: 10.3390/ani11041070

Figure Lengend Snippet: Antibodies, specificity and immunohistochemical procedure used.

Article Snippet: CD 206 , MR5D3 , Rat, Monoclonal , Macrophages (mannose receptor) , PTLink/pH6/20’ , 1:100 , Gene Tex, USA.

Techniques: Immunohistochemical staining, Marker, Clinical Proteomics, Plasmid Preparation